Shivani+Thakker

My idea for a project is based on the Lab 11 from AP Biology where we transformed E. coli with the pGLO plasmid, but instead of making E. coli resistant to ampicillin, I was wondering if other plasmids can transform the E. coli to become less pathogenic because that would be more relevant for us and more beneficial than making E. coli resistant to antibiotics.

The reason you made the E.coli resistant to the antibiotic is to be able to easily see that it was transformed. The pGlo lab is used to demonstrate transformation to students. It is not done for any practical purpose. You have to have an idea in which you can test an independent variable related to transformation. Do some more research and see what you come up with. Transformation is something we can do though

I thought about what Mrs. Palffy had talked to me about, and here are my ideas: to look for the rate of transformation in //E. coli, Giardia intestinalis, Trypanosoma, Plasmodium vivax, E. histolytica//

experiment could be: 3 to 5 bacteria, 1 plasmid with its genes known (and expected outcomes) tested for the rate of transformation by adding only 1 loopful of plasmid and study for 1 day, 2 days, and 3 days each.

OR

1 bacteria, 1 plasmid, tested for the rate of transformation by adding different amounts of plasmid but for only 1 day